Protective Effects of Total Flavonoids from Lysimachia christinae on Calcium Oxalate-Induced Oxidative Stress in a Renal Cell Line and Renal Tissue

Oxidative stress (OS) in renal tubular epithelial cells (RTECs) is induced by calcium oxalate (CaOx) stones and plays an important role the pathology of CaOx nephrolithiasis. The nuclear factor-E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway endogenous antioxidant pathway. Flavonoids are compounds with 2-phenylchromone as basic mother nucleus natural components Lysimachia christinae. Our previous studies demonstrated that total flavonoids from L. christinae (TFL) reduced oxalic acid concentrations urine, thus inhibiting stone formation. We also showed TFL can reduce OS tissue. However, whether inhibit formation through Nrf2/ARE requires further investigation. Here, we found protected against injury to a cell line tissue, CaOx-induced crystal In addition, significantly increased Nrf2 expression downstream genes heme oxygenase 1 (HO-1) NAD(P)H quinone oxidoreductase (NQO-1). Furthermore, superoxide dismutase (SOD) activity decreased malondialdehyde (MDA) content, thereby alleviating RTECs. Silencing blocked protective effect on OS. Taken together, our findings indicate tissue activating